Assay of succinate dehydrogenase activity in 60 wells by measuring formazan absorbance normalized to SDHB immunofluorescence.
Cultured astrocytes were stained with a classical histological staining for SDH activity and the amounts of the formazan product were quantified
by calculating optical density images and averaging the absorption in the perinuclear areas based on Hoechst 33342 staining. The picture shows calculated the optical
density image in red overlaid by the detected perinuclear areas in various colors. Montage of 60 wells.
From: (32).
Calibrated mitochondrial membrane potential painted on the insulin immunofluorescence image of human pancreatic β-cells. The "Image Output" feature of the Membrane Potential Calibration Wizard was used to create an image of ΔψM in mV, calculated for individual, whole cells. Then the above intensity gated (modulated) image was created by modulating the mV image with the intensity of insulin immunofluorescence. From: (26).
ATeam ratio in pyruvate-stimualted INS-1 832/13 cells. Image filtering and intensity gated (modulated) display was used in Image Analyst MKII to generate this movie. From: (16).
Fura-red ratio in pyruvate-stimualted INS-1 832/13 cells. Image filtering and intensity gated (modulated) pseudocolor display was used in Image Analyst MKII to generate this movie. From: (16).
INS-1 832/13 cells. Overlay of Fura-red and ATeam and a nuclear marker. From: (16).
ΔψP and ΔψM measurement in dispersed cultures of rat cortical neurons. Red, TMRM; green, a bis-oxonol plasma membrane potential indicator. The time lapse was recorded for calculation of mitochondrial and plasma membrane potentials in absolute millivolts. From: (17).
Fura-4F recording in rat dispersed neuronal cultures displayed in intensity gated pseudocolor using Image Analyst MKII. From: (9).
Mitochondrial Ca2+ signaling in brain capillary endothelial cells. Fluorescence time lapse of X-rhod-1 fluorescence during purinergic receptor stimulation using ATP. See high pass filtered version of this recording (next image). From: (1).
Mitochondrial Ca2+ signaling in brain capillary endothelial cells. Fluorescence time lapse of X-rhod-1 fluorescence during purinergic receptor stimulation using ATP. Images were high pass filtered using Image Analyst MKII. This allows separation of mitochondrially originated fluorescence from the bulk. From: (1).
Montage of a Seahorse microplate recording. All cells were counted in each well using image segmentation in Image Analyst MKII. This served to normalize Seahorse Extracellular Flux Analyzer oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) data. Note: the montage was created in Mathematica using images saved by Image Analyst MKII.
Reactive Oxygen Species assay using MitoSOX:MitoTracker Deep Red ratioing in microplates. The MitoSOX:MitoTracker Deep Red ratio is shown in pseudocolor as a montage of a 96-well microplate. Debris was removed from the images using image segmentation to improve the sensitivity of the assay. Note: the montage was created in Mathematica using images saved by Image Analyst MKII.
Reactive Oxygen Species assay using MitoSOX:MitoTracker Deep Red ratioing in microplates. The MitoSOX:MitoTracker Deep Red ratio is shown in pseudocolor in the center of a single well of a 96-well microplate. Debris was removed from the images using image segmentation to improve the sensitivity of the assay.
Tracks of overnight migration of human fibroblasts. The image is a montage from a 96-well microplate. Note: the montage was created in Mathematica using images saved by Image Analyst MKII.