Fluorescence microscopy image analysis
automation - time series - physiology

Materials available for downloading

• Reactive oxygen species assays
  1. MitoSOX:MitoTracker Deep Red ratio end point (105 MB)      Protocol
  2. DHE OH-ethidium:blue fluorescence ratio (32 MB)              Protocol
  3. DCFDA assay with internal calibration (99 MB)                  Protocol    
• Plasma and mitochondrial membrane potential calibration in human pancreatic β-cells sample data sets   
  1. Calibration of TMRM/FLIPR recordings (370 MB)      Protocol   Guide
  2. Calibration of TMRM/FLIPR recordings with post-hoc insulin immunocytochemistry (3 MB)  (This requires the above data, too.)   Guide
• Mitochondrial volume fractionator   
  • Using the basic pipeline with pancreatic β-cells (120 MB)     Protocol   Guide
• Mitochondrial swelling assay
  • Valinomycin-triggered mitochondrial swelling in rat cortical astrocytes (71 MB)
• FRET and [Ca2+]c assay for spectral unmixing
  • Using the calpain sensor pYSCS with Fura-6F in rat cortical neurons (35 MB)
• Mitochondrial motion assay sample data set recorded in rat hippocampal neurons using:
  • MetaMorph (107 MB)       Protocol
  • Elements (43 MB)          Protocol
  • Zeiss LSM 510 (27MB)    Protocol
• Mitochondrial NADH/NAD+ ratio with high pass filtering:
  • Two-photon microscopic recording of NAD(P)H autofluorescence in human fibroblasts (47 MB)    Protocol
    
• See more tutorials/protocols in the protocols page.